Staff Vitaliy B. Borisov

A. N. Belozersky Research Instituteof Physico-Chemical Biology MSU

  2. A. N. Belozersky Research Institute, MSU, Russia
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Vitaliy B. Borisov

Department
Department of Molecular Energetics of Microorganisms
Phone
+7(495)939-5149
Email
bor@belozersky.msu.ru

The group of Professor of the Russian Academy of Sciences (RAS), Ph.D., D.Sc., Principal Investigator Vitaliy B. Borisov is engaged in a comprehensive study of quinol oxidases of bacterial respiratory chains using modern biochemical and biophysical methods.

Main research directions

The main research directions include the elucidation of the molecular mechanism of functioning and the physiological role of quinol oxidases of bacterial respiratory chains (cytochromes bd and bo). These key components of the respiratory chains of prokaryotes can couple the oxidation of quinol by oxygen with the generation of proton-motive force. We are engaged in the identification of the unique features of quinol oxidases that allow them to combine energy conservation with the performance of other, alternative functions that are important for the survival, growth, and reproduction of bacterial cells. We are studying the resistance of quinol oxidases to a number of low-molecular-weight and physiologically active compounds; investigating the ability of enzymes to metabolize some of these compounds; elucidating the mechanisms of the generation of proton-motive force and the organization of cytochrome active sites for oxygen reduction; examining the main intermediates of the catalytic cycle of enzymes. New knowledge about the structural and functional properties of prokaryotic quinol oxidases and their differences from the properties of the terminal oxidase in humans and animals will contribute to the development of new pharmaceutical preparations against microbial infections in which these bacterial proteins serve as a therapeutic target.

Research results

Upon studies of bd-type quinol oxidases (E. coli cytochrome bd), for the first time, the rapid kinetics of membrane potential generation and sequential formation of spectrally distinguishable catalytic intermediates within one molecular turnover were recorded; data on the existence of an intraprotein proton-conducting pathway were obtained; femto-, pico-, and microsecond dynamics of enzyme's active site were established, and a fast electron transfer between the hemes was revealed; the affinity of heme d for oxygen was determined. It was found for the first time that the bd-type quinol oxidase endows E. coli with resistance to nitric oxide and hydrogen sulfide, and is also capable of metabolizing hydrogen peroxide and peroxynitrite.

Participation in joint research projects

Collaboration with the Sapienza University of Rome (Italy), the Kyushu Institute of Technology (Japan), the University of Sheffield (UK), the University of Illinois at Urbana-Champaign (USA), the University of Helsinki (Finland).

Grant support

The studies were supported by grants from the Russian Foundation for Basic Research (№ 99-04-48095, 02-04-48314, 05-04-48096, 08-04-00093, 11-04-00031, 14-04-00153, 19-04-00094).

Educational activities

Vitaliy B. Borisov is a lecturer of the annual course "Biochemistry" at the Faculty of Bioengineering and Bioinformatics of Lomonosov Moscow State University (since 2005).

Prizes

State Prize Award for young scientists (1999), Award of the Biochemical Society of Russia for young scientists (2000), Kaulen Award for young scientists for the best research in Belozersky Institute (2000), Award of the Russian Higher Education Academy of Sciences for young scientists (2000), Award for young scientists for the best research in Lomonosov Moscow State University (2001), Academia Europaea Prize for Young Russian Scientists (2002), Shuvalov Award of Lomonosov Moscow State University (2007).

Articles

  1. Borisov Vitaliy B., Forte E. (2025) Carbon Monoxide and Prokaryotic Energy Metabolism. International Journal of Molecular Sciences, >>

  2. Borisov Vitaliy B., Giardina Giorgio, Pistoia Gianluca, Forte E. (2025) Cytochrome bd-type oxidases and environmental stressors in microbial physiology. Advances in microbial physiology, >>

  3. Borisov Vitaliy B., Arutyunyan Alexander M. (2024) The fully reduced terminal oxidase bd-I isolated from Escherichia coli binds cyanide. Journal of Inorganic Biochemistry, >>

  4. Grivennikova Vera G., Gladyshev Grigory V., Zharova Tatyana V., Borisov Vitaliy B. (2024) Proton-Translocating NADH–Ubiquinone Oxidoreductase: Interaction with Artificial Electron Acceptors, Inhibitors, and Potential Medicines. International Journal of Molecular Sciences, >>

  5. Nastasi M.R., Borisov V.B., Forte E. (2024) Membrane-Bound Redox Enzyme Cytochrome bd-I Promotes Carbon Monoxide-Resistant Escherichia coli Growth and Respiration. International Journal of Molecular Sciences, >>

  6. Azarkina N.V., Borisov V.B., Oleynikov I.P., Sudakov R.V., Vygodina T.V. (2023) Interaction of Terminal Oxidases with Amphipathic Molecules. International Journal of Molecular Sciences, >>

  7. Борисов В.Б., Nastasi M.R., Forte E. (2023) Цитохром bd как антиоксидантный редокс-фермент. Молекулярная биология, >>

  8. Борисов В.Б. (2023) Генерация мембранного потенциала цитохромом bd. Биохимия, >>

  9. Borisov V.B. (2023) Generation of Membrane Potential by Cytochrome bd. Biochemistry (Moscow), >>

  10. Borisov V.B., Nastasi M.R., Forte E. (2023) Cytochrome bd as Antioxidant Redox Enzyme. Molecular Biology, >>

  11. Skulachev V.P., Vyssokikh M.Y., Chernyak B.V., Mulkidjanian A.Y., Skulachev M.V., Shilovsky G.A., Lyamzaev K.G., Borisov V.B., Severin F.F., Sadovnichii V.A. (2023) Six Functions of Respiration: Isn’t It Time to Take Control over ROS Production in Mitochondria, and Aging Along with It?. International Journal of Molecular Sciences, >>

  12. Nastasi M.R., Borisov V.B., Forte E. (2023) The terminal oxidase cytochrome bd-I confers carbon monoxide resistance to Escherichia coli cells. Journal of Inorganic Biochemistry, >>

  13. Zharova Tatyana V., Grivennikova Vera G., Borisov Vitaliy B. (2023) F1·Fo ATP Synthase/ATPase: Contemporary View on Unidirectional Catalysis. International Journal of Molecular Sciences, >>

  14. Форте Е., Настаси М.Р., Борисов В.Б. (2022) Препараты терминальной оксидазы цитохрома bd-II, выделенные из Escherichia coli, обнаруживают значительную активность в удалении перекиси водорода. Биохимия, >>

  15. Forte E., Nastasi M.R., Borisov V.B. (2022) Preparations of Terminal Oxidase Cytochrome bd-II Isolated from Escherichia coli Reveal Significant Hydrogen Peroxide Scavenging Activity. Biochemistry (Moscow), >>

  16. Borisov V.B., Forte E. (2022) Bioenergetics and Reactive Nitrogen Species in Bacteria. International Journal of Molecular Sciences, >>

  17. Friedrich T., Wohlwend D., Borisov V.B. (2022) Recent Advances in Structural Studies of Cytochrome bd and Its Potential Application as a Drug Target. International Journal of Molecular Sciences, >>

  18. Borisov V.B., Forte E. (2021) Impact of Hydrogen Sulfide on Mitochondrial and Bacterial Bioenergetics. International Journal of Molecular Sciences, >>

  19. Siletsky Sergey A., Borisov Vitaliy B. (2021) Proton Pumping and Non-Pumping Terminal Respiratory Oxidases: Active Sites Intermediates of These Molecular Machines and Their Derivatives. International Journal of Molecular Sciences, >>

  20. Борисов В.Б., Форте Е. (2021) ТЕРМИНАЛЬНАЯ ОКСИДАЗА ЦИТОХРОМ bd ЗАЩИЩАЕТ БАКТЕРИИ ОТ ТОКСИЧЕСКОГО ВОЗДЕЙСТВИЯ СЕРОВОДОРОДА. Биохимия, >>

  21. Borisov Vitaliy B., Siletsky Sergey A., Nastasi Martina R., Forte E. (2021) ROS Defense Systems and Terminal Oxidases in Bacteria. ANTIOXIDANTS, >>

  22. Borisov Vitaliy B., Forte E. (2021) Terminal Oxidase Cytochrome bd Protects Bacteria Against Hydrogen Sulfide Toxicity. Biochemistry (Moscow), >>

  23. Forte E., Siletsky Sergey A., Borisov Vitaliy B. (2021) In Escherichia coli Ammonia Inhibits Cytochrome bo3 But Activates Cytochrome bd-I. ANTIOXIDANTS, >>

  24. Siletsky Sergey A., Lukashev Evgeniy P., Mamedov Mahir D., Borisov Vitaliy B., Balashov Sergei P., Dolgikh Dmitriy A., Rubin Andrei B., Kirpichnikov Mikhail P., Petrovskaya Lada E. (2021) His57 controls the efficiency of ESR, a light-driven proton pump from Exiguobacterium sibiricum at low and high pH. Biochimica et Biophysica Acta - Bioenergetics, >>

  25. Borisov Vitaliy B., Siletsky Sergey A., Paiardini Alessandro, Hoogewijs David, Forte E., Giuffre Alessandro, Poole Robert K. (2021) Bacterial Oxidases of the Cytochrome bd Family: Redox Enzymes of Unique Structure, Function and Utility as Drug Targets. Antioxidants and Redox Signaling, >>