Department of Plant Proteins
Leader: Mikhail A. Belozersky
The department of Plant Proteins was organized in 2007 on the basis of the laboratory of plant proteins, which was part of the Department of Functional Biochemistry of Biopolymers of A.N. Belozersky Institute of Physico-Chemical Biology. Since its foundation to the present time it is headed by prof. M.A. Belozersky.
Research areas
(i) Plant defense mechanisms against phytopathogens and insect pests and development of approaches to increase plant tolerance to biotic stress.
(ii) Mechanisms of secretion and physiological role of hydrolytic enzymes of filamentous fungi
(iii) Mechanisms and organization of food proteins proteolysis in insects. Development of medicinal preparations for treatment of human digestive system disorders
The results improve our knowledge in the field of protein biochemistry and evolution, and could be used in biotechnology for development of new effective approaches to control of insect pests and in medicine for the development of drugs for treatment of human digestive system disorders.
International collaboration
During the last 15 years (1996-2012) the laboratory of Plant Proteins and formed in 2007 on its basis Department of Plant Proteins have been carrying out joint research work together with the U.S. Department of Agriculture (USDA ARS Grain Marketing and Production Research Center, Manhattan, Kansas), Biological Centre of the Academy of Sciences of Czech Republic (České Budeiovitse, Czech Republic), the Center of Biological Research of the Supreme Council of Research (Madrid, Spain), University of Milan (Milan, Italy), Institute of Genetics and Development of Cultivated Plants (Gatersleben, Germany), University of Fort Hare (South Africa), University of Copenhagen (Denmark).
Grants and projects
The research work in the Deparment was supported by RFBR individual grants, grants RFBR_Russia-Belarus, RFBR_Russia-Spain, RFBR_Russia-South Africa, RFBR_ofi_ts, Russian Ministry of Science _Russia-Italy, grants of the International Science and Technology Center (ISTC), U.S. Civilian Research and Development Foundation (CRDF), NATO-Russia Council, the Soros International Science Foundation.
Educational activities
Professor Y.E. Dunaevsky gives a course of lectures on biochemistry and physiology of fungi for the fourth year students at the Biological Faculty of Moscow State University. Under the supervision of the department researchers are run course, graduate and post-graduate dissertations of students from the Faculties of Bioengineering and Bioinformatics, Biological and Chemical Faculties of Moscow State University. Over the past five years (2006-2011), there were defended 4 Ph.D., 7 M.Sc. theses and over 15 term papers.
Research areas
- Plant defense mechanisms against phytopathogens and insect pests and development of approaches to increase plant tolerance to biotic stress.
- Mechanisms of secretion and physiological role of hydrolytic enzymes of filamentous fungi.
- Mechanisms and organization of food proteins proteolysis in insects. Development of medicinal preparations for treatment of human digestive system disorders.
(i) Plant defense mechanisms against phytopathogens and insect pests and development of approaches to increase plant tolerance to biotic stress.
(ii) Mechanisms of secretion and physiological role of hydrolytic enzymes of filamentous fungi
(iii) Mechanisms and organization of food proteins proteolysis in insects. Development of medicinal preparations for treatment of human digestive system disorders
The results improve our knowledge in the field of protein biochemistry and evolution, and could be used in biotechnology for development of new effective approaches to control of insect pests and in medicine for the development of drugs for treatment of human digestive system disorders.
International collaboration
During the last 15 years (1996-2012) the laboratory of Plant Proteins and formed in 2007 on its basis Department of Plant Proteins have been carrying out joint research work together with the U.S. Department of Agriculture (USDA ARS Grain Marketing and Production Research Center, Manhattan, Kansas), Biological Centre of the Academy of Sciences of Czech Republic (České Budeiovitse, Czech Republic), the Center of Biological Research of the Supreme Council of Research (Madrid, Spain), University of Milan (Milan, Italy), Institute of Genetics and Development of Cultivated Plants (Gatersleben, Germany), University of Fort Hare (South Africa), University of Copenhagen (Denmark).
Grants and projects
The research work in the Deparment was supported by RFBR individual grants, grants RFBR_Russia-Belarus, RFBR_Russia-Spain, RFBR_Russia-South Africa, RFBR_ofi_ts, Russian Ministry of Science _Russia-Italy, grants of the International Science and Technology Center (ISTC), U.S. Civilian Research and Development Foundation (CRDF), NATO-Russia Council, the Soros International Science Foundation.
Educational activities
Professor Y.E. Dunaevsky gives a course of lectures on biochemistry and physiology of fungi for the fourth year students at the Biological Faculty of Moscow State University. Under the supervision of the department researchers are run course, graduate and post-graduate dissertations of students from the Faculties of Bioengineering and Bioinformatics, Biological and Chemical Faculties of Moscow State University. Over the past five years (2006-2011), there were defended 4 Ph.D., 7 M.Sc. theses and over 15 term papers.
Articles
- Dunaevsky Ya E., Kudryavtseva O.A., Agroskin S.M., Gasparyan A.A., Belozersky M.A.(2024) Practical Achievements of Laboratory Evolution. Applied Biochemistry and Microbiology, >>
- Терещенкова В.Ф., Жиганов Н.И., Губаева А.С., Акентьев Ф.И., Дунаевский Я.Е., Козлов Д.Г., Белозерский М.А., Элпидина Е.Н.(2024) Рекомбинантная Химотрипсиноподобная Пептидаза Tenebrio molitor с Неканоническим Субстрат-связывающим Сайтом. Прикладная биохимия и микробиология, >>
- Zhiganov Nikita I., Vinokurov Konstantin S., Salimgareev Ruslan S., Tereshchenkova Valeriia F., Dunaevsky Yakov E., Belozersky Mikhail A., Elpidina Elena N.(2024) The Set of Serine Peptidases of the Tenebrio molitor Beetle: Transcriptomic Analysis on Different Developmental Stages. International Journal of Molecular Sciences, >>
- Tereshchenkova V.F., Zhiganov N.I., Gubaeva A.S., Akentyev F.I., Dunaevsky Y.E., Kozlov D.G., Belozersky M.A., Elpidina E.N.(2024) Recombinant Chymotrypsin-like Peptidase from Tenebrio molitor with a Non-Canonical Substrate-Binding Site. Applied Biochemistry and Microbiology, >>
- Dunaevsky Y.E., Elpidina E.N.(2024) Special Issue “Transcriptomics in the Study of Insect Biology". International Journal of Molecular Sciences, >>
- Акентьев Ф.И., Соколова Д.Д., Губайдуллин И.И., Рыков С.В., Березина О.В., Черноморова Н.О., Элпидина Е.Н., Козлов Д.Г.(2024) Увеличение скорости метаболизма сорбитола негативно влияет на метанол-индуцируемый синтез рекомбинантных ферментов в дрожжах Komagataella kurtzmanii и Komagataella phaffii. Биотехнология, >>
- Akentyev P.I., Sokolova D.D., Gubaidullin I.I., Rykov S.V., Berezina O.V., Chernomorova N.O., Elpidina E.N., Kozlov D.G.(2024) Increased Rate of Sorbitol Metabolism Negatively Affects Methanol-Induced Synthesis of Recombinant Enzymes in the Yeast Komagataella kurtzmanii and Komagataella phaffii. Applied Biochemistry and Microbiology, >>
- Kudryavtseva Olga A., Gerasimov Evgeny S., Glagoleva Elena S., Gasparyan Anna A., Agroskin Saveliy M., Belozersky Mikhail A., Dunaevsky Yakov E.(2023) Dynamics of Podospora anserina Genome Evolution in a Long-Term Experiment. International Journal of Molecular Sciences, >>
- Alkin N.A., Pokrovskaya Yu S., Belozerskii M.A., Kurakov A.V., Belyakova G.A., Dunaevskii Ya E.(2023) On the Presence of Gluten-Cleaving Activity in Sodiomyces alkalinus and S. magadiensis Strains. Doklady Biological Sciences, >>
- Tereshchenkova V.F., Filippova I.Y., Goptar I.A., Dunaevsky Y.E., Belozersky M.A., Elpidina E.N.(2023) Complex of Proline-Specific Peptidases in the Genome and Gut Transcriptomes of Tenebrionidae Insects and Their Role in Gliadin Hydrolysis. International Journal of Molecular Sciences, >>
- Chugunov Anton O., Dvoryakova Elena A., Dyuzheva Maria A., Simonyan Tatyana R., Tereshchenkova Valeria F., Filippova Irina Yu, Efremov Roman G., Elpidina Elena N.(2023) Fighting Celiac Disease: Improvement of pH Stability of Cathepsin L In Vitro by Computational Design. International Journal of Molecular Sciences, >>
- Serkina A.V., Gubaidullin I.I., Akentiev F.I., Dombrovskii I.A., Filippova I.Yu, Elpidina E.N., Kozlov D.G., Bulushova N.V.(2023) Development of the Fundamentals of a Technology for the Production of Tribolium castaneum Recombinant Cathepsin L in Komagataella kurtzmanii Yeast. Applied Biochemistry and Microbiology, >>
- Dvoryakova Elena A., Klimova Maria A., Simonyan Tatiana R., Dombrovsky Ivan A., Serebryakova M., Tereshchenkova Valeriia F., Dunaevsky Yakov E., Belozersky Mikhail A., Filippova Irina Y., Elpidina Elena N.(2022) Recombinant Cathepsin L of Tribolium castaneum and Its Potential in the Hydrolysis of Immunogenic Gliadin Peptides. International Journal of Molecular Sciences, >>
- Dvoryakova E.A., Vinokurov K.S., Tereshchenkova V.F., Dunaevsky Y.E., Belozersky M.A., Oppert B., Filippova I.Y., Elpidina E.N.(2022) Primary digestive cathepsins L of Tribolium castaneum larvae: Proteomic identification, properties, comparison with human lysosomal cathepsin L. Insect Biochemistry and Molecular Biology, >>
- Серкина А.В., Губайдуллин И.И., Акентьев Ф.И., Домбровский И.А., Филиппова И.Ю., Элпидина Е.Н., Козлов Д.Г., Булушова Н.В.(2022) Разработка основ технологии получения рекомбинантного катепсина L Tribolium castaneum в дрожжах Komagataella kurtzmanii. Биотехнология, >>
- Oppert B., Muszewska A., Steczkiewicz K., Šatović-Vukšić E., Plohl M., Fabrick J.A., Vinokurov K.S., … Chaply R.A., Elpidina E.N., Tereshchenkova V.F., … Campbell J.F.(2022) The Genome of Rhyzopertha dominica (Fab.) (Coleoptera: Bostrichidae): Adaptation for Success. Genes, >>
- Алкин Н.А., Покровская Ю.С., Белозерский М.А., Кураков А.В., Белякова Г.А., Дунаевский Я.Е.(2021) О ПРИСУТСТВИИ ГЛЮТЕНРАСЩЕПЛЯЮЩЕЙ АКТИВНОСТИ У ШТАММОВ SODIOMYCES ALKALINUS И S. MAGADIENSIS. Микология и фитопатология, >>
- Dunaevsky Yakov E., Tereshchenkova Valeriia F., Belozersky Mikhail A., Filippova Irina Y., Oppert Brenda, Elpidina Elena N.(2021) Effective Degradation of Gluten and Its Fragments by Gluten-Specific Peptidases: A Review on Application for the Treatment of Patients with Gluten Sensitivity. Pharmaceutics, >>
- Alkin Nikita, Dunaevsky Yakov, Elpidina Elena, Beljakova Galina, Tereshchenkova Valeria, Filippova Irina, Belozersky Mikhail(2021) Proline-Specific Fungal Peptidases: Genomic Analysis and Identification of Secreted DPP4 in Alkaliphilic and Alkalitolerant Fungi. Journal of Fungi, >>
- Zhiganov Nikita I., Tereshchenkova Valeriia F., Oppert Brenda, Filippova Irina Y., Belyaeva Nataliya V., Dunaevsky Yakov E., Belozersky Mikhail A., Elpidina Elena N.(2021) The dataset of predicted trypsin serine peptidases and their inactive homologs in Tenebrio molitor transcriptomes. Data in Brief, >>
- ЛАВРЕНТЬЕВА Е.В., ЭРДЫНЕЕВА Е.Б., ДУНАЕВСКИЙ Я.Е., БОЛТЯНСКАЯ Ю.В., КЕВБРИН В.В.(2021) ВНЕКЛЕТОЧНЫЕ ВЫСОКОСТАБИЛЬНЫЕ ЩЕЛОЧНЫЕ ПЕПТИДАЗЫ АЛКАЛОФИЛЬНЫХ БАКТЕРИЙ ALKALICAULIS SATELLES И ALIIDIOMARINA SP., ПЕРСПЕКТИВА ИХ ПРИМЕНЕНИЯ В СОСТАВЕ ДЕТЕРГЕНТОВ. Прикладная биохимия и микробиология, >>
- Жиганов Н.И., Терещенкова В.Ф., Bandani Ali Reza, Филиппова И.Ю., Элпидина Е.Н.(2021) Сериновые пептидазы семейства S1 в геномах и транскриптомах тенебрионид – вредителей запасов зерновых культур. Актуальная биотехнология, >>
- Gorbunov A.A., Akentyev F.I., Gubaidullin I.I., Zhiganov N.I., Tereshchenkova V.F., Elpidina E.N., Kozlov D.G.(2021) Biosynthesis and Secretion of Serine Peptidase SerP38 from Tenebrio molitor in the Yeast Komagataella kurtzmanii. Applied Biochemistry and Microbiology, >>
- Tereshchenkova V.F., Zhiganov N.I., Akentyev P.I., Gubaidullin I.I., Kozlov D.G., Belyaeva N.V., Filippova I.Yu, Elpidina E.N.(2021) Preparation and Properties of the Recombinant Tenebrio molitor SerPH122—Proteolytically Active Homolog of Serine Peptidase. Applied Biochemistry and Microbiology, >>
- Терещенкова В.Ф., Жиганов Н.И., Акентьев Ф.И., Губайдуллин И.И., Козлов Д.Г., Беляева Н.В., Филиппова И.Ю., Элпидина Е.Н.(2021) Получение и свойства рекомбинантного протеолитически активного гомолога сериновой пептидазы SerPH122 Tenebrio molitor. Прикладная биохимия и микробиология, >>